|
KMID : 0380020190340030173
|
|
Korean Journal of Biotechnology and Bioengineering
2019 Volume.34 No. 3 p.173 ~ p.177
|
|
|
Enhancing Lipid Production in the Arctic Microalga Chlamydomonas sp. KNM0029C by Using Statistical Optimization of Medium Components
|
|
Kim Eun-Jae
Jung Woong-Sic
Kim Sang-Hee
Choi Han-Gu
Han Se-Jong
|
|
|
Abstract
|
|
|
|
A sequential statistical optimization was applied to the components of Tris-acetate-phosphate (TAP) medium, to enhance the cell density and lipid production of Arctic microalga Chlamydomonas sp. KNM0029C. The evaluation was preformed through an elimination process and Plackett-Burman design to select for significant factors, contributing toward cell growth. NH4Cl, MgSO4·7H2O, potassium phosphate, and trace elements were established as the effective components and used as variable factors in Box-Behnken design test, - a response surface methodology (RSM). The final optimized concentrations were 0.54, 0.16, 0.04, and 0.08 g/L of NH4Cl, MgSO4·7H2O, potassium phosphate, and trace elements, respectively. Overall, 9.9% enhanced lipid production was achieved by using optimized TAP medium at 4°C. The results of the present study could potentially contribute toward large-scale lipid production at low temperatures.
|
|
|
KEYWORD
|
|
|
arctic microalgae, Chlamydomonas sp. KNM0029C, lipid, response surface methodology, statistical optimization, tris?acetate-phosphate
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|